Pdf experimental pathogenicity of shrimp, penaeus vannamei. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. Domestication of black tiger shrimp penaeusmonodon in recirculation systems in vietnam. The prevalence of viral pathogens white spot syndrome virus wssv, hepatopancreatic parvo virus hpv, monodon baculo virus.
A baculovirus disease of penaeus monodon fabricius named monodon baculovirus mbv was found in. Current status of viral diseases in asian shrimp aquaculture. The simultaneous presence of monodon baculovirus mbv and whitespot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by nested polymerase chain reaction pcr. Some occur not only in cultivated shrimp in other asian countries, but also in those from australia and the western hemisphere. Mbv virions are rodshaped, nonoccluded or occluded. Synthethic peptide used to develop antibodies for detection. They were reared for one year to become f 0 pondreared. Two recombinant baculovirus vectors with shrimp viral promoters wssvie1 and ihhnvp2 were constructed bacie1gfp and bacp2gfp and green fluorescent protein gfp used as the transgene. Pdf prevalence of white spot syndrome virus wssv and. Baculoviruses are known to infect invertebrates, with over 600 host species having been described. An evidence on transovarian transmission of monodon baculovirus mbv infection. There appear to be more ob when pl are stressed, so the presence of ob can be taken as an indication of stress in the pl. Penaeid shrimp, viral disease, monodon baculovirus, mbv. There are several advantages of using baculovirus expression system over e.
Aug 01, 2008 monodon baculovirus mbv of penaeid shrimp has been associated with high mortalities in hatchery. Mbv is pathogenic to several species of shrimp such as p. An evidence on transovarian transmission of monodon baculovirus. Diagnosis of penaeus monodon type baculovirus by pcr and by elisa of occlusion bodies. Detection of monodon baculovirus and whitespot syndrome virus. In contrast, the bactobac baculovirus expression system is faster and easier because. Hsu yl, wang kl, yang mc, hu ch, lo cf, wang ch and hsu t 2000. Hence, it is essential to maintain good water quality by increased water exchange in order to minimise risks of secondary bacterial infections cumulatively leading to mortality. Pdf among the viruses infecting penaeid shrimp, monodontype baculovirus mbv otherwise. The monodon baculovirus mbv also known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv was the first shrimp viral pathogen reported from asia. These products are not produced or registered in the u. This will reduce the risk of pathogen introduction by this route, as well as increase overall security.
Status of shrimp diseases and advances in shrimp health. Millions of metric tons dominated by penaeus monodon major change to penaeus vannamei year world shrimp production culture. However, only 15% of samples were positive by nonnested reaction. The israeli journal of aquaculture bamidgeh 6, 2009 fig. This cell line is susceptible to and capable of mass producing virus, which. Penaeus monodon mature and breed only in tropical marine habitats and spend their larval, juvenile, adolescent and subadult stages in coastal estuaries, lagoons or mangrove areas. White spot baculovirus syndrome in the indian shrimp penaeus. It was first reported in laboratory reared adult p. We report the use of recombinant baculovirus mediated transduction using hybrid promoter system for transgene expression in lymphoid cells of penaeus monodon. Recently, a number of advances have been made in the characteriza tion of the replication of baculovirus dna, including the identification.
The author and the promoters give the authorisation to consult and tocopy. The plasmid pvl93 is a baculovirus expression vector for the expression of genes with an atg nformyl methionine translation initiation codon. Monodon baculovirus mbv disease of penaeid shrimp dfompo. Aquaculture elsev1er aquaculture l 995 1295 a record and prevalence omonodon baculovirus from postlarval penaeus monodon in madras, india p. Monodon baculovirus of shrimp article pdf available. Besides mbv, two strains of snpv are reportedplebejus. White spot baculovirus syndrome in the indian shrimp. Monodon baculovirus mbv is a major virus that infects the hepatopancreas of cultivated shrimp species lightner 1996 and may cause growth retardation at late stages of cultivation flegel et al. World production of shrimp from capture fisheries and aquaculture.
Baculodirect baculovirus expression system makes baculovirus expression more convenient, requiring less hands on time than traditional systems. Monodon baculovirus of shrimp europe pmc article europe pmc. Monodon baculovirus mbv causes slow growth of the shrimp being due to its. It is a rodshaped, enveloped, doublestranded dna virus, and considered till recently, as the type a baculovirus. The cytoplasm revealed increased numbers of free ribosomes, proliferation of. Detection of monodon baculovirus and whitespot syndrome. There are currently 76 species in this family, divided among four genera.
The objective of our study was to explain the histopathological changes of monodon baculovirus mbv in hepatopancreas and midgut tissues of the cultured penaeus vannamei. For customers who prefer a eukaryotic expression system, are exploring lowercost alternatives to mammalian expression system but. Traditional baculovirus expression systems utilize tedious and time consuming sitespecific transposition in e. Freepenaeus monodon hatchery manual based on the experience in india. Prevalence of white spot syndrome virus and monodon baculovirus in penaeus monodon broodstock and postlarvae from hatcheries in southeast coast of india. There are currently 84 species in this family, divided among four genera. Penaeus monodon, nonoccluded baculovirus nob ii wongteerasupaya et al.
Environmental assessment report import of specific. Improving penaeus monodon hatchery practices, a manual based on the shrimp farming experience in india. Embedded virions are released and fuse to microvillar epithelial cells. The monodon baculovirus mbv also known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv was the first shrimp viral pathogen reported from asia rajendran et al.
Investigation of monodontype baculovirus mbv infection in penaeus monodon. First incidence of looseshell syndrome disease in the. Guide to baculovirus expression vector systems bevs and. Baculodirect baculovirus expression system thermo fisher. Ultrastructural and pathogenesis of monodon baculovirus in spf transmission electron microscopy tem study on prepared samples of shrimps exposing to the virus.
Virions free in the nuclei and within the occlusion bodies measure about. Among the viruses infecting penaeid shrimp, monodontype baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the. This cell line is susceptible to and capable of mass producing virus, which includes, but not limited to, white spot syndrome virus wssv, panaeus monodon baculovirus mbv, infectious hypodermal and hematopoietic necrosis virus ihhnv, and infectious pancreatic necrosis. Research article strain variations in monodon baculovirus.
Penaeus monodon is the secondmost widely cultured prawn species in the world, after only whiteleg shrimp, litopenaeus vannamei. Manual of histologic staining methods of the armed forces institute of pathology. Fastgrowing and whitespot syndrome virus wssvfree shrimp were selected from commercial growout ponds. Penaeus monodon baculovirus mbv in cultured jstage. Mbv monodon baculovirus shrimp care aquaculture products. Mbv was first identified in 1977 from taiwanese shrimps 47. Aug 25, 2012 among the viruses infecting penaeid shrimp, monodontype baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. Vertical transmission after the ov is ingested by insect larvae, the crystalline. The simultaneous presence of monodon baculovirus mbv and whitespot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by. Penaeus monodon baculovirus mbv is a rodshaped virus possessing a relatively thin. Production was dominated by penaeus monodonuntil 2002 and by. Bactobac baculovirus expression system thermo fisher. This will reduce the risk of pathogen introduction by this route, as well as. Traditional baculovirus systems require purification and amplification of an initial lowtiter viral supernatant.
Domestication and selective breeding of penaeus monodon in. Major viral diseases of the black tiger prawn penaeus. Vertical transmission after the ov is ingested by insect larvae, the crystalline polyhedrin matrix is degraded in the alkaline midgut of the insect. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported. Baculovirus expression vector system bd biosciences. Pdf prevalence of white spot syndrome virus and monodon. Monodon baculovirus, penaeus vannamei, intranuclear. Pdf production of monoclonal antibodies to polyhedrin of.
These f 1 offspring were screened to be free of wssv before. Translation initiation sequences must be provided by the. Domestication of black tiger shrimp ugent pages 1 50. Monodon baculovirus mbv exists in populations of the indian tiger prawn, penaeus monodon, obtained from a commercial hatchery in madras, india. A record and prevalence of monodon baculovirus from. Observations on the geographic distribution, pathogenesis and. Even though mbv is not a severe pathogen for the black tiger prawn penaeus monodon, it should be eliminated from the farming system because it is unlikely that. Overview of the baculovirus expression system chambers. Jayakumar1 unit of cell biology and parasitology, department of zoology, life science building, university of madras, madras 600 025, india school of biology and biochemistry, medical biology centre, the. Spherical baculovirosis penaeus monodon type baculovirus p. Monodon baculovirus mbv of penaeid shrimp has been associated with high mortalities in hatchery.
Each operational unit should have sufficient area and perimeter to permit free passage and convenient working conditions. Environmental assessment report import of specific pathogen. Production was dominated by penaeus monodonuntil 2002 and by penaeus vannamei thereafter. Us6143547a cell line derived from penaeus monodon and a. In the wild, they show marked nocturnal activity, burrowing into bottom substratum during the day and emerging at night to search for food as benthic feeders.
Infected with a baculovirus which resembled monodon baculovirus mbv in its. In 2009, 770,000 tonnes were produced, with a total value of. Manual based on experience in india of animals and unauthorized persons. The present invention relates to the establishment of an immortal shrimp cell line pmo derived from penaeus monodon. Mbv virions are rodshaped, nonoccluded or occluded and enveloped within a paracrystalline occlusion body. Some of these viruses cause disease in other penaeid shrimp species and even other crustacean species. This paradigm switch in the species being farmed occurred within 5 years after the first spf p. Mbv monodon baculovirus the occlusion bodies ob formed as monodon baculovirus mbv replicate are easily visible using a microscope.
Arthropods, lepidoptera, hymenoptera, diptera, and decapoda serve as natural hosts. Production of monoclonal antibodies to polyhedrin of monodon baculovirus mbv from shrimp chumpol boonsanongchokying 1, wiwat sangoum 1, paisarn sithigorngul 2, siriporn sriurairatana 1. Prevalence of white spot syndrome virus wssv and monodon baculovirus mbv infection in penaeus monodon postlarvae in vietnam. The baculovirus expression vector system the baculovirus expression vector system bevs is one of the most powerful and versatile eukaryotic expression systems available. Experimental pathogenicity of shrimp, penaeus vannamei exposed. Occurrence of viral pathogens in penaeus monodon post.
Ultrastructural and pathogenesis of monodon baculovirus in. Aug 25, 2012 among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. First incidence of looseshell syndrome disease in the giant. Manual of histological staining methods of the armed forces. There appear to be more ob when pl are stressed, so the. Ultrastructural and pathogenesis of monodon baculovirus in spf transmission electron microscopy tem study on prepared samples of shrimps exposing to the virus was continued for transmission electron microscopy following afsharnasab 2007. Development and evaluation of a dot blot analysis for the. Baculovirus dna replication 857 p10, which encodes a small poorly conserved protein that may be in volved in occlusion body formation or cell lysis van oers et al. There are five different viruses which are currently being studied for their impact on commercial farming of the black tiger prawn penaeus monodon in thailand. White spot baculovirus syndrome in the indian shrimp penaeus monodon and p.
Baculovirus expression systems typically require bacterial transformation and isolation of a large bacmid or cotransfection of a transfer vector and linear baculovirus dna into insect cells. Development of sybr green based real time pcr assay for. Washing eggs and nauplii with disinfectant did not free monodon baculovirus mbv infection from postlarvae. Mbv is one of the widely reported and well described viral pathogens of shrimp.
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